Studies Involving Multidrug Resistant Salmonella

Multiple drug resistant(MDR) Salmonella enterica serovar Typhimurium phage type DT104 was virtually non-existent in the early 1990's. Since that time, it has rapidly disseminated worldwide.  This strain is resistant to core group of antimicrobials including ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline (commonly abbreviated ACSSuT), however, isolates have been identified which are also resistant to fluoroquinolones, trimethoprim and kanamycin.  Case control studies have suggested that MDR DT104 is more virulent than sensitive strains of DT104, however, attempts to demonstrate MDR DT104 is more invasive in vitro have been unsuccessful. Additional serotypes which have acquired this genomic island have caused additional outbreaks.

All of the resistance genes are located on a 44 kb region of the chromosome which has been labelled Salmonella Genomic Island 1(SGI1). 

Publications related to SGI1 from work conducted in our laboratory

1. Mulvey, MR, D.A. Boyd, A.B. Olson, B. Doublet, A. Cloeckaert. The genetics of Salmonella genomic island 1, Microbes Infect. 8 (2006). In press.[REVIEW]

2. Doublet D., D. Boyd, M. R. Mulvey, and A. Cloeckaert. 2005. The Salmonella genomic island 1 is an integrative mobilizable element.  Molecular Microbiology. 55:1911-1924.

3. Baucheron S., S. Tyler, D. Boyd, M. R. Mulvey, E. Chaslus-Dancla, A. Cloeckaert .  2004. AcrAB-TolC directs efflux-mediated multidrug resistance in Salmonella enterica serovar typhimurium DT104 Antimicrob Agents Chemother. 48:3729-3735.

4. Mulvey, M. R., D. Boyd, A. Cloeckaert, R. Ahmed and L-K Ng, and the Canadian Public Health Laboratories. 2004.  A dramatic increase of Salmonella enterica Serovar Paratyphi B dT+ harboring the antibiotic resistant Salmonella Genomic Island 1 in Canada in 2001-2002. Emerging Infectious Diseases. 10:1307-1310.

5. Doublet, B., P. Butaye, H. Imberechts, D. Boyd, M. R. Mulvey, E. Chaslus-Dancla, and A. Cloeckaert.  2004.  Characterization of Salmonella genomic island 1 multidrug resistance gene clusters from S. enterica serovar Agona, Belgium, 1992-2002. Antimicrob. Agents Chemother. 48:2510-2517.

6. Doublet, B., R. Lailler, D. Meunier, A. Brisabois, D. Boyd, M. R. Mulvey, E. Chaslus-Dancla, and A. Cloeckaert. 2003. Identification of a variant Salmonella genomic island I antibiotic resistance gene cluster in Salmonella enterica serovar Albany.  Emerging Infectious Diseases. 9:585-591.

7. Boyd, D., A. Cloeckaert, E. Chaslus-Dancla, and M. R. Mulvey.  2002.  Characterization of Variant Salmonella Genomic Island 1 Multidrug Resistance Regions from Serovars Typhimurium DT104 and Agona.  Antimicrobial Agents and Chemotherapy.  46:1714-1722.

8. Meunier, D., D. Boyd, M. R. Mulvey, S. Baucheron,C. Mammina, A. Nastasi, E. Chaslus-Dancla, and A Cloeckaert. 2002  Salmonella enterica Serovar Typhimurium DT104 antibiotic resistance Genomic Island I in S. enterica Serovar Paratyphi B.  Emerging Infectious Diseases.  8:430-433.

9. Boyd, D., G. Peters, A. Cloeckaert, K. Boumedine, E. Chaslus-Dancla, H. Imberechts, and M. Mulvey. 2001.  Complete nucleotide sequence of a 43 kb genomic island associated with the multidrug resistance region of Salmonella enterica Typhimurium DT104 and its identification in phage type DT120 and serovar Agona.  Journal of Bacteriology. 183:5725-5732.

10. Boyd, D A., G. A. Peters, L-K. Ng, and M. R. Mulvey. 2000. Partial characterization of a genomic island associated with the multidrug resistance region of Salmonella enterica Typhimurium DT104.FEMS Microbiology Letters. 189:285-291.

11. Ng, L-K, M. R. Mulvey, I. Martin, G. A. Peters, and W. Johnson. 1999. Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar Typhimurium DT104.  Antimicrobial Agents and Chemotherapy. 43:3018-3021.