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Human Metapneumovirus (HMPV)

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Reference Diagnostic Test
Requisition Form

Molecular Diagnostic of Respiratory Viruses (PDF document - 1095 KB - 1 page)

Reference Details
Reference Diagnostic Test:
Molecular Detection by PCR or RT-PCR
Test Description:

Molecular Detection by PCR or RT-PCR for Human Metapuenmovirus (HMPV).

Pathogen:
Human Metapneumovirus (HMPV)
Laboratory:
Influenza and Respiratory Viruses
Illnesses & Diseases:
Respiratory tract infections
Test Category:
Molecular Detection
Specimen:

Respiratory specimens (nasopharyngeal swab, throat swab, other). Volume required: 1-2 mL.

Collection method:

Use sterile swabs and swab nasal cavity, throat, etc. Place swabs in 2-3 mL viral transport medium.

Specimen Processing and Storage:

Keep samples refrigerated until shipped for testing. Ship on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Suspected or confirmed viral respiratory infection.

Accompanying Documentation:

Completed requisition (current Molecular Diagnostic for Respiratory Viruses requisition form). Sender lab name, address, and telephone number must be included for samples to be processed. Also include identifier (sender lab number), patient date of birth, patient gender, type of specimen, and date collected. Under additional information, please include if possible/applicable: passage history, patient travel history, extent of influenza activity, and date of collection relative to antiviral use

Comments:

Authorization must be obtained for molecular detection by PCR or RT-PCR for Human Bocavirus (HBoV), Adenovirus, Human Coronavirus (HCoV), Human Rhinovirus, Influenza virus, Respiratory Syncytial Virus (RSV), Human ParaInfluenza virus. Please contact the Influenza and Respiratory Viruses laboratory by phone or email before sending specimens.

Method and Interpretation of Results:

Nucleic acids are extracted from submitted respiratory specimens. Polymerase Chain Reaction (PCR) or Reverse Transcriptase (RT) PCR with virus-specific primers is performed on extracted nucleic acids to determine the presence of viruses (using real-time detection or visual confirmation by gel electrophoresis of PCR products).

Turnaround time:

14 calendar days

Contact:
Influenza and Respiratory Viruses
Phone: (204) 789-6045
Fax: (204) 789-2082
Email: Yan.Li@phac-aspc.gc.ca
References :
Fact Sheet:
Related Information: