Public Health Agency of Canada
Symbol of the Government of Canada

Common menu bar links

Molecular Detection of Mycobacterium tuberculosis Complex (MTBC) from Clinical Samples

Back to Tests List

Reference Diagnostic Test
Requisition Form

Mycobacterium tuberculosis Complex Detection from Direct Patient Samples (PDF document - 901 KB - 1 page)
National Reference Centre for Mycobacteriology Requisition (PDF document - 707 KB - 1 page)

Reference Details
Reference Diagnostic Test:
Molecular Detection of Mycobacterium tuberculosis Complex (MTBC) from Clinical Samples
Test Description:

Real-time PCR based detection of MTBC in clinical samples using various molecular targets.

Test Category:
Molecular Detection
Pathogen:
Mycobacterium tuberculosis
Laboratory:
National Reference Centre for Mycobacteriology
Illnesses & Diseases:
Tuberculosis (TB)
Specimen:

Please consult with the NRCM Program Manager/designate to determine if your sample is appropriate for this type of testing.

Collection method:

Clinical samples such as, but not restricted to, fresh tissues or sediment from decontaminated samples.

Specimen Processing and Storage:

Ship clinical specimens such as sputum or fresh tissue on ice for overnight delivery, and prior to Wednesday each week to ensure receipt by Friday. Previously frozen material can be shipped on dry ice using appropriate Transportation of Dangerous Goods shipping procedures. Please consult the NRCM if further information is required.

 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Please consult with the NRCM prior to submitting a sample for MTBC detection.

Accompanying Documentation:
  • Special request only. Test request must be made with prior consultation with NRCM manager/designate.
  • Justification by physician in-charge using the form titled "Request Form for Mycobacterium tuberculosis Complex Detection from Direct Patient Samples" must be included with the NRCM requisition form.
  • Requisitions, along with justification, must be faxed prior to shipping isolates to NRCM - attention Joyce Wolfe, Program Manager, NRCM/NML at 204-789-2036. Requisition for the NRCM and the Request Form for Mycobacterium tuberculosis Complex Detection from Direct Patient Samples must be included with submission. The Requisition for the NRCM should be completed and signed off by the supervisor/designate and physician in charge of the submitting laboratory and include the source of specimen, patient gender, date of birth, clinical history, submitting laboratory identifier and submitter information microscopy if available) and any other information relevant to the submitted sample. The Request Form for Mycobacterium tuberculosis Complex Detection from Direct Patient Samples should be filled out completely and signed off by the physician in charge    
Comments:

Processed or clinical sample requirements are outlined on the Request Form for Mycobacterium tuberculosis Complex Detection from Direct Patient Samples. Samples may be rejected if a low volume of sample is submitted, or appropriate documentation and justification is incomplete or missing.

 

Method and Interpretation of Results:

A variety of molecular methods such as real-time PCR for the detection of IS6110 and RD9 may be employed for testing.

Turnaround time:

Within 2 working days.

Contact:
National Reference Centre for Mycobacteriology
Phone: (204) 789-6038
Fax: (204) 789-2036
Email: NML.NRCM@phac-aspc.gc.ca
References :

1. Eisenach KD, Cave MD, Bates JH, and Crawford JT. (1990) Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J. Infect. Dis. 161:977-981.

2. Huard RC, de Oliveira Lazzarini LC, Butler WR, van Soolingen D, and Ho JL. (2003) PCR-Based Method To Differentiate the Subspecies of the Mycobacterium tuberculosis Complex on the Basis of Genomic Deletions. J. Clin. Microbiol. 41:1637.

Fact Sheet: MYCOBACTERIUM TUBERCULOSIS COMPLEX - PATHOGEN SAFETY DATA SHEET
Related Information: Case Definitions for Communicable Diseases under National Surveillance - 2009 - Tuberculosis
Tuberculosis FACT SHEETS
Tuberculosis Prevention and Control